Kuzma told team officials Saturday morning that the ankle was sore, and it was decided to hold him out for precautionary reasons.
He tallied 12 points in the Americans' 102-86 win over Australia on Thursday, going 4-of-5 from 3-point range.
It is unclear whether Kuzma will be available Monday when the U.S. finishes its pre-World Cup exhibition tour in Sydney against Canada.
Also on Saturday, Marcus Smart of the Boston Celtics saw his first action of the team's exhibition season and started against the Australians. Smart has been slowed for about two weeks by a strained left calf.