Police said the charge stems from an incident on Friday when Gladney allegedly assaulted a 22-year-old woman.
"We are aware of Jeff's arrest and are gathering additional information," the Vikings said in a statement Monday. "We take this matter very seriously, as the reported allegations are extremely disturbing. At this time we will have no further comment."
Gladney, 24, was a first-round draft pick out of TCU in 2020 and started 15 games for Minnesota last season.