The Lions didn't practice Monday but he -- and receiver Marvin Jones Jr. (knee) -- were estimated as being non-participants in practice.
The rookie, who has been one of the Lions' most dynamic players this season, injured the knee on a run where he started heading right, saw defenders and cut all the way back to the left side of the field before being tackled near the sideline for a 3-yard gain.
Johnson got up, went to the sideline and was immediately looked at by Lions staff, first on the bench and then on the medical table behind the bench before heading to the locker room for further examination.
Johnson entered Sunday's game with 103 carries for 554 yards and two touchdowns along with 30 catches for 203 yards and a touchdown. Prior to the injury, Johnson had 15 carries for 87 yards and a touchdown along with two catches for 10 yards against the Panthers.
ESPN's Michael Rothstein contributed to this report.