England duo Sam Underhill and Ben Te'o decided against wearing rainbow laces against Australia at Twickenham on Saturday due to comfort issues.
The Rugby Football Union (RFU) allowed the England players to choose whether they wanted to wear the laces this weekend but both Underhill and Te'o opted against it.
"I won't be wearing them but I fully support the LGBT community," Underhill said.
"That's something we are all very, very keen that people know.
"They are actually really uncomfortable in my boots - and they are really long."
Te'o, 31, said: "I haven't got the laces at the moment. I'll just leave my boots as they are, as I've had them for the autumn."
Both sets of England and Australia players were given the chance to make an individual decision on the laces before the Test match at Twickenham.